Welcome to the Light-Sheet Imaging at Universidad Mayor –LiSIUM– Initiative! Light-sheet microscopy is a powerful approach for fast and gentle 3D-imaging of intact living model organisms, tissues, and developing cells. At Universidad Mayor (UM), we have obtained (with FONDEQUIP) the only streamlined model in the region –a ZEISS Light-sheet 7 microscope– raising significant interest among our collaborators seeking to implement light-sheet bioimaging. Moreover, we have recently received funding from the Chan Zuckerberg Initiative to Expand Global Access to Bioimaging, through which we aim to connect several labs at the Universidad Mayor (UM) with other institutions in Chile and international labs to build a hub for light-sheet microscopy in Latin America.